Reexamining Polyphenol Oxidase, Peroxidase, and Leaf-blackening Activity in Protea
نویسندگان
چکیده
منابع مشابه
Peroxidase-polyphenol oxidase association in Dioscorea esculenta.
A crude enzyme extract from Dioscorea esculenta var. fasiculata tissue subjected to ion exchange chromatography on DEAE-Sephadex A-50 column. This procedure resolved the extract into two main protein peaks one of which eluted through the column relatively unbound while the other protein peak which remained bound to the column was eluted with 1.0 M NaCl. Both protein peaks contained polyphenol o...
متن کاملPOLYPHENOL OXIDASE ACTIVITY AND DIFFERENTIATION OF RHIZOMORPH IN ARMILLARIA MELLEA (VAML. EX. FR.) KARST
Polyphenol oxidase (PPO) activity varied in differentiation from hyphae to rhizomorph and croute in A. mellea. In undifferentiated hyphae there was not any PPO activity, while croute showed the highest activity. Electrophoresis in the presence of SDS allows the detection of intensive enzyme forms of PPO, and can also convert slower migrating enzyme forms to faster ones. Some fluorescent co...
متن کاملPolyphenol oxidase activity expression in Ralstonia solanacearum.
Sequencing of the genome of Ralstonia solanacearum revealed several genes that putatively code for polyphenol oxidases (PPOs). To study the actual expression of these genes, we looked for and detected all kinds of PPO activities, including laccase, cresolase, and catechol oxidase activities, in cellular extracts of this microorganism. The conditions for the PPO assays were optimized for the phe...
متن کاملDirect Electrochemistry of Polyphenol Oxidase
The electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNTs) is presented. Cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (PPO). A redox couple with an anodic and counterpart cathodic peak is obtained. The influence of various parameters such as pH,...
متن کاملInvestigations of Metals and Metal-Antibrowning Agents Effects on Polyphenol Oxidase Activity from Red Poppy Leaf
Heavy metals are one of the major groups of contaminants in the environment and many of them are toxic even at very low concentration in plants and animals. However, some metals play important roles in the biological function of many enzymes in living organisms. Metals such as zinc, iron, and cooper are important for survival and activity of enzymes in plants, however heavy metals can inhibit e...
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ژورنال
عنوان ژورنال: Journal of the American Society for Horticultural Science
سال: 1994
ISSN: 0003-1062,2327-9788
DOI: 10.21273/jashs.119.6.1248